human pancreatic ductal epithelial cells hpde  (ATCC)

Journal: Translational Cancer Research

Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells

doi: 10.21037/tcr-2025-1050

Figure Lengend Snippet: Effect of tormentic acid on the proliferation of PDAC cells. (A) Chemical structure of tormentic acid. Viability of (B) PANC-1, (C) MIA PaCa-2 and (D) HPDE cells treated with various concentrations of tormentic acid, measured by the CCK-8 assay. (E) Phase contrast images of PANC-1 cells showed morphological changes following treatment with tormentic acid. Experiments were performed in triplicate, and data are presented as mean ± standard deviation. Images captured at ×20 magnification. *, P<0.05. CCK-8, Cell Counting Kit-8; PDAC, pancreatic ductal adenocarcinoma.

Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.

Techniques: CCK-8 Assay, Standard Deviation, Cell Counting

Journal: Heliyon

Article Title: Circular RNA circTMEM59 inhibits progression of pancreatic ductal adenocarcinoma by targeting miR-147b/SOCS1: An in vitro study

doi: 10.1016/j.heliyon.2024.e24402

Figure Lengend Snippet: CircTMEM59 was downregulated in PDAC cells and tissues. (A) The expression of circTMEM59 in PDAC tumor tissues and normal tissues was analyzed by GEO database; (B) GEO database showed that circTMEM59 was remarkably decreased in PDAC tissues; ** p < 0.01 vs. NC; (C) The circTMEM59 expression in PDAC tumor tissues and adjacent normal tissues was measured by qRT-PCR; ** p < 0.01 vs. NC; (D) Kaplan-Meier analysis of overall survival of PDAC patients according to circTMEM59 expression levels; (E) The expression levels of circTMEM59 in PDAC cell lines (SW1990, PANC-1, BXPC-3 and CFPAC-1) and human pancreatic ductal epithelial (HPDE) cells were measured by qRT-PCR; * p < 0.05 vs. HPDE, ** p < 0.01 vs. HPDE; (F) CircTMEM59 and linear TMEM59 expression levels were detected after RNase R treatment in PDAC cells (PANC-1 and BXPC-3); ** p < 0.01 vs. mock. PDAC, pancreatic ductal adenocarcinoma.

Article Snippet: A total of 4 PDAC cell lines (PANC-1, CFPAC-1, SW1990 and BXPC-3) as well as human normal pancreatic ductal epithelial (HPDE) cells were obtained from the ATCC cell bank (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: Heliyon

Article Title: Circular RNA circTMEM59 inhibits progression of pancreatic ductal adenocarcinoma by targeting miR-147b/SOCS1: An in vitro study

doi: 10.1016/j.heliyon.2024.e24402

Figure Lengend Snippet: Overexpression of circTMEM59 inhibited proliferation, invasion and EMT of PDAC cells. (A) After transfection with circTMEM59, the relative circTMEM59 in PANC-1 and BXPC-3 cell lines were detected by qRT-PCR; ** p < 0.01 vs. control; (B) Cell proliferation was detected by CCK8 assay in cells transfected with vector or circTMEM59; ** p < 0.01 vs. vector; (C) Cell invasion was detected by Transwell assay in cells transfected with vector or circTMEM59; ** p < 0.01 vs. vector; (D) The morphology of transfected cells were observed by a microscope; (E) The expression levels of PCNA, E-cadherin and vimentin were detected by Western blot analysis in cells transfected with vector or circTMEM59; ** p < 0.01 vs. vector. EMT, epithelial-mesenchymal transition; PDAC, pancreatic ductal adenocarcinoma; PCNA, proliferation cell nuclear antigen.

Article Snippet: A total of 4 PDAC cell lines (PANC-1, CFPAC-1, SW1990 and BXPC-3) as well as human normal pancreatic ductal epithelial (HPDE) cells were obtained from the ATCC cell bank (Shanghai, China).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Control, CCK-8 Assay, Plasmid Preparation, Transwell Assay, Microscopy, Expressing, Western Blot

Journal: Heliyon

Article Title: Circular RNA circTMEM59 inhibits progression of pancreatic ductal adenocarcinoma by targeting miR-147b/SOCS1: An in vitro study

doi: 10.1016/j.heliyon.2024.e24402

Figure Lengend Snippet: Knockdown of miR-147b inhibited proliferation, invasion and EMT of PDAC cells. (A) After transfection with miR-147b inhibitors, the relative miR-147b in PANC-1 and BXPC-3 cell lines were detected by qRT-PCR; ** p < 0.01 vs. control; (B) Cell proliferation was detected by CCK8 assay in cells transfected with NC inhibitors or miR-147b inhibitors; ** p < 0.01 vs. NC inhibitor; (C) Cell invasion was detected by Transwell assay in cells transfected with NC inhibitors or miR-147b inhibitors; ** p < 0.01 vs. NC inhibitor; (D) The morphology of transfected cells were observed by a microscope; (E) The expression levels of PCNA, E-cadherin and vimentin were detected by Western blot analysis in cells transfected with NC inhibitors or miR-147b inhibitors; ** p < 0.01 vs. NC inhibitor. EMT, epithelial-mesenchymal transition; PDAC, pancreatic ductal adenocarcinoma; PCNA, proliferation cell nuclear antigen. ** p < 0.01.

Article Snippet: A total of 4 PDAC cell lines (PANC-1, CFPAC-1, SW1990 and BXPC-3) as well as human normal pancreatic ductal epithelial (HPDE) cells were obtained from the ATCC cell bank (Shanghai, China).

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Control, CCK-8 Assay, Transwell Assay, Microscopy, Expressing, Western Blot