CCK-8 Assay:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Standard Deviation:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Cell Counting:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Expressing:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Mass Spectrometry:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Tandem Mass Spectroscopy:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Labeling:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Fluorescence:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Transfection:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Western Blot:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Activity Assay:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Inhibition:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Knockdown:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
Concentration Assay:Article Title: A pentacyclic triterpene tormentic acid inhibits the proliferation and migration of pancreatic ductal adenocarcinoma cells
Article Snippet: Human PDAC cell lines PANC-1 (ATCC ® CRL-1469TM, RRID:CVCL_0480) and MIA PaCa-2 (ATCC ® CRM-CRL-1420TM, RRID:CVCL_0428), along with normal human pancreatic ductal epithelial cells HPDE (HPDE6-C7, RRID:CVCL_4376), were obtained from the American Type Culture Collection (ATCC) and relevant sources.. All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2 .All cell lines are of human origin (Homo sapiens) and were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, and 1% L-glutamine at 37 °C in a humidified incubator containing 5% CO 2..
Article Title: The pancreatitis-cancer transformation-related factor, human rhomboid family-1, promotes pancreatic cancer progression through the SRC/YAP signaling pathway
Article Snippet: CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.CP-PDAC tumor samples and paired adjacent non-tumor samples from 11 patients were subjected to transcriptome sequencing.. Human PDAC cells (Capan-1, MiaPaCa-2, SW1990, PANC-1, and BxPC-3), human pancreatic ductal epithelial cells (HPDE), human pancreatic stellate cells (HPSCs), and cancer-associated fibroblasts (CAFs) were obtained from the American Type Culture Collection.. The cells were cultured as previously described [ , ].The cells were cultured as previously described [ , ].
Article Title: Comprehensive pan-cancer analysis of role of GPRASP1, associated with clinical outcomes, immune microenvironment, and immunotherapeutic efficiency in pancreatic cancer.
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were purchased from the ATCC.. Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).Human pancreatic cancer cells PANC-1, MIA PaCa-2, BxPC-3, SW1990, and AsPC-1 were obtained from Procell (Wuhan, China), Biobaiye (Shanghai, China), and BIOON (Shanghai, China).
Article Title: Proteome and secretome analysis of pancreatic cancer cells.
Article Snippet: In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.In addition, some key protein changes were confirmed via parallel reactionmonitoring (PRM)mass spectrometry.. Four pancreatic cancer cell lines obtained from human pancreatic ductal adenocarcinomas (PANC-1, PaCa-44, MIA PaCa-2, and BxPC3), as well as normal human pancreatic ductal epithelial cells (HPDE) (CRL-4023) were obtained from the American Type Culture Collection (ATCC,Manassas, VA).. Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.Thedetailedprocedures for proteinpreparation from the cellular proteome and the secretome are described in SupplementaryMethods.
Article Title: Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14
Article Snippet: Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.Cell culture, cell strains, and human patient samples Parental pancreatic cell lines.. Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario, Canada), and human primary tumorderived cells (Panc10.05), human metastatically-derived cells (AsPC-1), and human-derived HeLa cells were purchased from ATCC.. The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.The Panc4.03 cell line was established in the Jaffee lab. All were grown using standard cell culture methods.
Article Title: Harnessing the adaptive potential of mechanoresponsive proteins to overwhelm pancreatic cancer dissemination and invasion
Article Snippet: Human pancreatic ductal epithelial cells (HPDE) were obtained from Dr. Ming-Sound Tsao (University of Toronto, Ontario Canada) and human primary tumor-derived cells (Panc10.05) and human metastatically-derived cells (AsPC-1) were purchased from ATCC.. Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .Panc02 cells (highly tumorigenic murine pancreatic tumor cell line) were derived from a methylcholanthrenetreated C57B1/56 mouse , .
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